H4777

Heparan sulfate proteoglycan

≥400 μg/mL glycosaminoglycan

• Sinónimos: HSPG
• Número MDL: MFCD00283416
• Código UNSPSC: 12352202
• NACRES: NA.75

Propiedades

• origen biológico: mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
• Nivel de calidad: 300
• descripción: For cell culture use.; Isolated from basement membrane of Engelbreth-Holm-Swarm mouse sarcoma.; Solution in 50 mM Tris HCl, 150 mM NaCl, 1 mM EDTA, 0.1 mM PMSF, pH 7.4, containing ≥400 μg protein per ml.
• Formulario: solution
• mol peso: ≥400 kDa
• envase: pkg of 0.1 mg
• concentración: ≥400 μg/mL glycosaminoglycan
• técnicas: cell culture | mammalian: suitable
• impurezas: ≥100 μg/mL Uronic acid
• Nº de acceso NCBI: NM_008305.4
• Nº de acceso UniProt: Q05793
• Especificidad de unión: Peptide Source: Collagen Type IV; ; Peptide Source: Fibronectin; ; Peptide Source: Laminin
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: mouse ... Hspg2(15530)

Descripción

Descripción general

Heparan sulfate proteoglycan (HSPG), a glycoprotein, is an integral part of the basement membrane. It is composed of a core protein covalently bound to heparan sulfate chains. There are three groups of HSPGs: membrane HSPGs, secreted extracellular matrix HSPGs, and the secretory vesicle proteoglycan.

Aplicación

Heparan sulfate proteoglycan has been used:

• in phosphate buffered saline (PBS) and added to fresh citrated blood samples to achieve trauma-equivalent concentrations in a research study
• solid phase binding assay
• for fabrication of extracellular matrix (ECM) microarray slides

Acciones bioquímicas o fisiológicas

Heparan sulfate proteoglycans (HSPGs) have multiple functions in various biological processes. They contribute to basement membrane structure and provide a matrix for cell migration. HSPGs in secretory vesicles, such as serglycin, play a role in packaging granular contents, maintaining proteases activity, and regulating biological activities after secretion. HSPGs can bind and protect cytokines, growth factors, and chemokines, facilitating the formation of gradients essential for cell specification and leukocyte recruitment. They act as receptors for proteases and protease inhibitors, regulate cell-ECM attachment, cell-cell interactions, and cell motility, and can cooperate with growth factor receptors. HSPGs also function as endocytic receptors for ligand clearance and are involved in lipoprotein metabolism and morphogen gradient formation. It also acts as a docking molecule for matrilysin and other matrix metalloproteinases. Heparan Sulfate promotes the attachment of cells in culture and induces high-affinity binding of FGF-basic to cells deficient in heparin sulfate and soluble FGF receptors at a concentration of 10-100 ng/mL.

Componentes

Heparan sulfate proteoglycan is a large biomolecule composed of a core protein covalently bound to heparin sulfate chains that is an integral part of the basement membrane. The number of polysaccharide chains and the size of the core protein are variable according to the source.

Precaución

It is recommended to store this product at 20°C, where it will retain its function for 3 years

Nota de preparación

This product is isolated from the basement membrane of Engelbreth-Holm-Swarm mouse sarcoma in a solution containing 50 mM Tris HCl at pH 7.4, 150 mM NaCl, 1 mM EDTA, and 0.1 mM PMSF.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)