ABS2183M

Anti-Coagulation factor VIIa

from rabbit, purified by affinity chromatography

• Sinónimos: Coagulation factor VII, EC: 3.4.21.21, Proconvertin, Serum prothrombin conversion accelerator, SPCA
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): primate (based on 100% sequence homology)
• envase: antibody small pack of 25 μg
• técnicas: immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_000122
• Nº de acceso UniProt: P08709
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... F7(2155)

Descripción

Descripción general

Coagulation factor VII (UniProt: P08709; also known as EC: 3.4.21.21, Proconvertin, Serum prothrombin conversion accelerator, SPCA) is encoded by the F7 gene (Gene ID: 2155) in human. Coagulation factor VII is a member of the peptidase S1 family that initiates the extrinsic pathway of blood coagulation. It is serine protease that is synthesized with a signal peptide (aa 1-20) and a propeptide (aa 21-60) and circulates in the blood in a zymogen form. The mature form is a heterodimer of a light and a heavy chain. It is a vitamin K-dependent protease that binds to calcium following enzymatic carboxylation of some glutamate residues. Factor VII is converted to Factor VIIa by the action of Factor Xa, Factor XIIa, Factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, Factor VIIa converts factor X to factor Xa by cleavage of Arg-|-Ile bond. Factor VIIa also converts factor IX to factor IXa in the presence of tissue factor and calcium. Factor VII deficiency resulting from mutations in F7 gene can lead to hemorrhagic complications.

Especificidad

This rabbit polyclonal antibody detects coagulation factor VIIa in human.

Inmunógeno

Full-length human recombinant Coagulation factor VIIa.

Aplicación

Anti-Coagulation factor VIIa Antibody, Cat. No. ABS2183, is a rabbit polyclonal antibody that detects Coagulation factor VIIa and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Immunofluorescence Analysis: A representative lot detected Coagulation factor VIIa in endothelial cell cultures incubated with rFVIIa (6 mg/mL) for 2 h at 37˚C.

Immunocytochemistry Analysis: A representative lot detected Coagulation factor VIIa in umbilical cord veins.

Calidad

Evaluated by Western Blotting in human placental tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Coagulation factor VIIa in 10 µg of human placental tissue lysate.

Descripción de destino

~57 kDa observed; 51.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Otras notas

Concentration: Please refer to lot specific datasheet.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable