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Key Documents

AB5320C3

Sigma-Aldrich

Anti-NG2 Chondroitin Sulfate Proteoglycan, Cy3 Conjugate Antibody

from rabbit, CY3 conjugate

Sinónimos:

Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

conjugado

CY3 conjugate

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

reactividad de especies

rat

reactividad de especies (predicha por homología)

mouse (based on 100% sequence homology), monkey (based on 100% sequence homology), human (based on 100% sequence homology)

técnicas

immunocytochemistry: suitable
immunohistochemistry: suitable

isotipo

IgG

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... CSPG4(1464)
mouse ... Cspg4(121021)
rat ... Cspg4(81651)
rhesus monkey ... Cspg4(713086)

Descripción general

NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.

Especificidad

This antibody identifies the intact proteoglycan and core protein by WB and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.

Inmunógeno

Immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat

Aplicación

Anti-NG2 Chondroitin Sulfate Proteoglycan, Cy3 Conjugate Antibody is an antibody against NG2 Chondroitin Sulfate Proteoglycan for use in ICC & IHC.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Calidad

Evaluated by Immunocytochemistry in rat oligodendrocyte precursor cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NG2 in rat oligodendrocyte precursor cells. Performed without Triton.

Descripción de destino

The unconjugated parent antibody (Cat. No. AB5320) has an observed molecular weight at ~270-300 kDa

Forma física

Protein A purified
Purified rabbit polyclonal conjugated to Cy3 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Almacenamiento y estabilidad

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Nota de análisis

Control
Rat or Human oligodendrocyte precursor cells

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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