71129

pETcoco-1 DNA - Novagen

Novagen′s pETcoco-1 expression vector is designed to allow "on command" amplification of vector copy number from single copy (1-2 plasmid copies per cell) to medium copy (20-50 per cell).

• Código UNSPSC: 41106602
• NACRES: NA.51

Propiedades

• etiqueta: Herpes Simplex Virus (HSV) tagged; His tagged; S-Tag tagged
• Nivel de calidad: 100
• Formulario: liquid
• fabricante / nombre comercial: Novagen^®
• condiciones de almacenamiento: OK to freeze
• Condiciones de envío: wet ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

The pETcoco-1 expression vector is designed to allow "on command" amplification of vector copy number from single copy (1–2 plasmid copies per cell) to medium copy (20–50 per cell). The vector is maintained in the single copy state by utiliziing the oriS and repE elements of the F episome together with the parABC partition determinants. The medium copy state is obtained by employing the RK-2 derived trfA replicator acting at oriV. The switch from single copy to medium copy status is achieved by inducing the expression of the trfA gene with arabinose. The dual replicon nature of these vectors promotes enhanced stability of recombinant plasmids and extremely low basal expression levels in the single copy state, while allowing robust target gene expression typical of the pET vectors. Unique restriction sites are shown on the circle map. pETcoco-1 contains the chloramphenicol resistance gene. The sequence is numbered relative to the start of T7 transcription at +1.

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.

Componentes

•10 µgpETcoco-1 DNA

•0.2 mlInduction Control Glycerol Stock

Advertencia

Toxicity: Standard Handling (A)

Otras notas

Vector Family: pETcoco

Información legal

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable