70856

RNase A Solution

Convenient solution for selective degradation of RNA

• Sinónimos: RNase A Enzyme
• Código UNSPSC: 12352202
• NACRES: NA.54

Propiedades

• formulario: solution
• fabricante / nombre comercial: Novagen^®
• condiciones de almacenamiento: OK to freeze
• concentración: 10 mg/mL
• técnicas: DNA purification: suitable
• Condiciones de envío: ambient
• temp. de almacenamiento: 10-30°C

Descripción

Descripción general

RNase A Solution is a convenient alternative to powdered RNase A. It is a highly purified preparation of bovine pancreatic ribonuclease A suitable for use in selective removal of RNA. It has been pretreated to remove DNase I and is suitable to remove RNA contamination during plasmid and genomic DNA purification procedures. Supplied at a concentration of 10 mg/ml in 10 mM Tris-HCl, 1 mM EDTA, 50% glycerol, pH 7.5.

Aplicación

RNase A Solution has been used:

• in cell cycle profiling of embryonic mouse neuroectodermal cells (NE-4C)
• in the digestion of RNA for extraction of DNA from human osteosarcoma cell lines for chromatin immunoprecipitation (ChIP) assays
• to treat control adult and embryonic thymi cells during staining
• to remove RNA from affibody–mRNA fusion clones before clone screening

Acciones bioquímicas o fisiológicas

RNaseA is an endoribonuclease that catalyzes the degradation of phosphodiester linkages with a pyrimidine base at the 3′ -position on single-stranded RNA via, transphosphorylation and hydrolysis mechanism.

Advertencia

Toxicity: Standard Handling (A)

Información legal

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2