393206
Anti-HNE Fluorophore Rabbit pAb
liquid, Calbiochem®
Sinónimos:
Anti-N α-Acetyllysine-4-hydroxy-2-nonenal Fluorophore, Anti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.43
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
diluted serum
tipo de anticuerpo
primary antibodies
clon
polyclonal
Formulario
liquid
reactividad de especies (predicha por homología)
all
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
avoid repeated freeze/thaw cycles
isotipo
IgG
Condiciones de envío
wet ice
temp. de almacenamiento
−70°C
modificación del objetivo postraduccional
unmodified
Descripción general
Anti-HNE Fluorophore, rabbit polyclonal, recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore. It is validated for use in ELISA, Western blotting, and immunohistochemistry.
Rabbit polyclonal antibody supplied as diluted serum. Recognizes the HNE-derived chromophore of the 2:1 lysine-HNE fluorophore.
Recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore.
Inmunógeno
a 2:1 Nα-acetyllysine-HNE fluorophore, conjugated to KLH
Aplicación
ELISA (1:500-1:1000)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500)
Advertencia
Toxicity: Standard Handling (A)
Forma física
Serum diluted 1:1 with glycerol.
Reconstitución
Following initial thaw, aliquot and freeze (-70°C).
Otras notas
Due to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.
Tsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 7975.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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