2384

Creatine Phosphokinase, Rabbit Skeletal Muscle

• Sinónimos: Creatine Phosphokinase, Rabbit Skeletal Muscle, rCPK
• Número de CAS: 9001-15-4
• Comisión internacional de enzimas: 2.7.3.2 (BRENDA, IUBMB)
• Número MDL: MFCD00130876
• Código UNSPSC: 12352202
• NACRES: NA.21

Propiedades

• origen biológico: rabbit skeletal muscle
• Nivel de calidad: 200
• Formulario: lyophilized
• actividad específica: ≥300 U/mg, 25 °C; 750 U/mg, 37 °C
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; desiccated (hygroscopic)
• solubilidad: 0.1 M imidazole buffer, pH 6.6: soluble; 0.5% NaHCO₃, pH 9.0: soluble
• actividad extraña: ATPase, hexokinase, myokinase <0.001%
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Native creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.

Reconstitución

Following reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.

Otras notas

Bessman, S.P. 1987. Anal. Biochem.161, 519.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable