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Merck

C3755

Sigma-Aldrich

Creatine Phosphokinase from rabbit muscle

Type I, salt-free, lyophilized powder, ≥150 units/mg protein

Sinónimos:

ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número CE:
Número MDL:
Código UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54

tpo

Type I

Nivel de calidad

formulario

salt-free, lyophilized powder

actividad específica

≥150 units/mg protein

mol peso

80-86.2 kDa

solubilidad

0.25 M glycyl-glycine, pH 7.4: soluble 5.0 mg/mL, clear, colorless to slightly yellow

actividad extraña

ATPase ≤0.01%
Lactic dehydrogenase, hexokinase, myokinase and pyruvate kinase ≤0.001%

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

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Aplicación

Molecular Weight: ~81,000
Creatine Phosphokinase is a dimer composed predominantly of the skeletal muscle derived homodimer (MM). CK also exists as a heterodimer (MB) particularly in the myocardium. CK derived from brain tissue consists mainly of the brain source homodimer (BB). The amino acid sequences of the M chain and B chains are about 80% homologous. From the sequence, the molecular weight of the M chain is 43,112.

E1%(280)= 8.76

pH Optimum: pH 8.8-9.0 for the forward reaction and pH 6.0-7.0 for the reverse reaction.

CK is a cellular enzyme with a wide tissue distribution. Its physiological role is associated with ATP generation for contractile or transport systems. Increased levels of CK are associated with myocardial infarction, muscular dystrophy, hyperthyroidism, pulmonary infarction and cerebrovascular disease. Variations in relative isozyme distribution can provide additional information in the diagnosis of these conditions.

Substrates: Creatine, N-ethylglycocyamine and glyocyamine have been shown to act as substrates for CK. CK is very specific for ATP/ADP.

Inhibitors: ADP is a strong inhibitor of the forward reaction competing with ATP. Divalent cations such as Ca2+ (Ki=4.5 mM), Zn2+ and Cu2+ inhibit CK by competing with Mg2+. Other inhibitors include acetate, acetylsalicylic acid, adenosine, p-aminosalicylic acid, AMP, benzoic acid, bicarbonate, bromide, chloride, p-Chloromercuribenzoic acid, ethylene oxide, 2,4-fluorodinitrobenzene, iodide, malonic acid, NAD, nitrate, phosphate, pyrophosphate, salicylic acid, sulfate, sulfite, thyroxine, trichloroacetate, L-triiodothyroxine, L-triiodothyronine, and tripolyphosphate.
The enzyme from Sigma has been used in the measurement of phosphocreatine in hippocampal neurons isolated from rat brains.

Precaución

The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution.

Definición de unidad

One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C.

Nota de preparación

Produces a clear, colorless to light yellow solution at 5 mg/mL in 0.25 M glycyl-glycine, pH 7.4

Nota de análisis

Protein determined by biuret.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

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Mitochondrial creatine kinase: a key enzyme of aerobic energy metabolism.
M Wyss et al.
Biochimica et biophysica acta, 1102(2), 119-166 (1992-09-25)
G J Brewer et al.
Journal of neurochemistry, 74(5), 1968-1978 (2000-05-09)
The loss of ATP, which is needed for ionic homeostasis, is an early event in the neurotoxicity of glutamate and beta-amyloid (A(beta)). We hypothesize that cells supplemented with the precursor creatine make more phosphocreatine (PCr) and create larger energy reserves
M Kliffen et al.
Laboratory investigation; a journal of technical methods and pathology, 73(2), 267-272 (1995-08-01)
Age-related maculopathy (ARM) is the most common cause of blindness in the elderly in the western world. Its early stage is characterized by many histopathologic changes, including two extracellular deposits, basal laminar deposit (BLD) and drusen. The origin and chemical
Jean-Claude Tardif et al.
Journal of the American College of Cardiology, 61(20), 2048-2055 (2013-03-19)
The study aimed to evaluate inclacumab for the reduction of myocardial damage during a percutaneous coronary intervention (PCI) in patients with non-ST-segment elevation myocardial infarction. P-selectin is an adhesion molecule involved in interactions between endothelial cells, platelets, and leukocytes. Inclacumab
Nina Bögershausen et al.
American journal of human genetics, 93(1), 181-190 (2013-07-09)
Myopathies are a clinically and etiologically heterogeneous group of disorders that can range from limb girdle muscular dystrophy (LGMD) to syndromic forms with associated features including intellectual disability. Here, we report the identification of mutations in transport protein particle complex

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