234109-M

Colcemid

Cell synchronization agent.

• Sinónimos: Colcemid, Demecolcine
• Fórmula empírica (notación de Hill): C₂₁H₂₅NO₅
• Número de CAS: 477-30-5
• Peso molecular: 371.43
• Número MDL: MFCD00075459
• Código UNSPSC: 12352200

Propiedades

• descripción: Merck USA index - 14, 2887
• Ensayo: ≥98% (HPLC)
• Formulario: crystalline powder
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• solubilidad: chloroform: 10 mg/mL; ethanol: soluble
• temp. de almacenamiento: 10-30°C
• cadena SMILES: N(C1CCc2c(c(c(c(c2)OC)OC)OC)C3=CC=C(C(=O)C=C31)OC)C
• InChI: 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3
• Clave InChI: NNJPGOLRFBJNIW-UHFFFAOYSA-N

Descripción

Descripción general

Cell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate the spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.

Acciones bioquímicas o fisiológicas

Primary Target
Depolymerizes microtubules and limits microtubule formation.

Advertencia

Toxicity: Toxic & Carcinogenic / Teratogenic (G)

Reconstitución

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 6 months at -20°C.

Otras notas

Urbani, L., et al. 1995. Exp. Cell Res. 219, 159.
Kanda, R., et al. 1994. J. Radiat. Res. 35, 41.
Sherwood, S.W., et al. 1994. Exp. Cell Res. 215, 373.
Nishiyama, I., and Fuji, T. 1992. Exp. Cell Res.198, 214.
Spoerri, P.E., and Roisen, F.J. 1992. J. Neurosci. Res.31, 494.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany