07-1601

Anti-IRAK1 Antibody, CT

from rabbit

• Sinónimos: interleukin-1 receptor-associated kinase 1, Pelle homolog
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: human
• técnicas: western blot: suitable
• Nº de acceso NCBI: NP_001020413.1
• Nº de acceso UniProt: P51617
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... IRAK1(3654)

Descripción

Descripción general

IRAK1, also known as Interleukin-1 receptor associated kinase 1, belongs to the tyrosine kinase related (TKL) family and is involved with mRNA stabilization and activation of NF-kappa B. IRAK1 is located in all tissue as 2 isoforms, with 1 binding quickly and then degrading while isoform 2 is activated by kinase, resulting in a slower more sustained response to cytokines. Phosphorylation of IRAK1 leads to recruitment of the adapter protein PELI1. IRAK1 utilizes 2 pathways, the first one allows for mediation of IL-1 inflammatory responses when TRAF6 is bound, which activates NF-kappa B, thereby inhibiting I kappa B alpha. A second pathway does not involve TRAF6 and results in the stabilization of mRNA.

Especificidad

This antibody recognizes the C-terminal region of IRAK1.

Inmunógeno

Epitope: C-terminus

KLH-conjugated synthetic linear peptide corresponding to the C-terminal region of IRAK1.

Aplicación

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

This Anti-IRAK1 Antibody, C-terminus is validated for use in WB for the detection of IRAK1.

Calidad

Evaluated by western blot in jurkat lysate.

Western Blot Analysis: A 1:50-1:500 dilution of this antibody detected IRAK1 on jurkat lysate.

Descripción de destino

~77 kDa

Forma física

Format: Purified

Protein G Purified

Purified rabbit polyclonal in buffer containing PBS and 0.09% sodium azide.

Almacenamiento y estabilidad

Stable for 6 months at 2-8ºC from date of receipt. For long term storage, store at -20ºC.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
Jurkat lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable