07-582-I

Anti-phospho-IRF-3 (Ser396) Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Interferon regulatory factor 3, IRF-3
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• técnicas: western blot: suitable
• Nº de acceso NCBI: NP_001562
• Nº de acceso UniProt: Q14653
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pSer396)
• Información sobre el gen: human ... IRF3(3661) ; pig ... Irf3(396656) ; rhesus monkey ... Irf3(718970)

Descripción

Descripción general

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.

Especificidad

This antibody recognizes IRF-3 phosphorylated at Ser396.

Inmunógeno

Epitope: SNS → ANA: Mutation of this residue results in loss of viral-induced phosphorylation of IRF-3.

KLH-conjugated linear peptide corresponding to human IRF-3 phosphorylated at Ser396.

Aplicación

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3.

Calidad

Evaluated by Western Blot in untreated and sendai virus treated A549 cell lysates.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-IRF-3 (Ser396) in 10 µg of sendai virus treated A549 cell lysate.

Descripción de destino

~50 kDa observed. Uncharacterized bands may appear at ~40 kDa and ~80 kDa in some lysates.

Ligadura / enlace

Replaces: 07-582

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Untreated and sendai virus treated A549 cell lysates

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable