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Documentos clave

07-164

Sigma-Aldrich

Anti-phospho-H2A.X (Ser139) Antibody

Upstate®, from rabbit

Sinónimos:

H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

rabbit

Nivel de calidad

100

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

polyclonal

reactividad de especies

human, vertebrates

envase

antibody small pack of 25 μg

fabricante / nombre comercial

Upstate®

técnicas

ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
western blot: suitable

isotipo

IgG

Nº de acceso NCBI

NP_002096

Nº de acceso UniProt

P16104

Condiciones de envío

ambient

modificación del objetivo postraduccional

phosphorylation (pSer139)

Información sobre el gen

human ... HIST2H2AC(8338)

Descripción general

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Especificidad

Broad species cross-reactivity expected based on conservation of sequence homology.
Histone H2A.X phosphorylated at Ser139.

Inmunógeno

Epitope: a.a. 134-142

Aplicación

Anti-phospho-H2A.X (Ser139) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-H2A.X (Ser139) also known as histone 2 H2ac, histone IIa & has been validated in IP & WB.
Immunoprecipitation:
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.

Calidad

Routinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.

Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.

Descripción de destino

15 kDa

Ligadura / enlace

Replaces: MABE205

Forma física

Format: Purified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Nota de análisis

Control
Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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