ABE347

Anti-NFκB p65 Antibody, CT

from rabbit

• Sinónimos: v-rel reticuloendotheliosis viral oncogene homolog A (avian), v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)), Nuclear factor of kappa light polypeptide gene enhancer
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: mouse, human, rat
• envase: antibody small pack of 25 μg
• técnicas: immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_068810
• Nº de acceso UniProt: Q04206
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... RELA(5970)

Descripción

Descripción general

The transcription factor NFκB (Nuclear Factor κB) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFκB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFκB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFκB is the most abundant in cells.

Especificidad

This antibody recognizes NFκB at the C-terminus.

Inmunógeno

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human NFκB.

Aplicación

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-NFκB p65 Antibody, CT (Rabbit Polyclonal Antibody) validated in WB, IP to detect NFκB p65 also known as transcription factor p65.

Western Blot (SNAP i.d.) Analysis: 5 µg/mL from a representative lot detected NFκB on 10 µg of A431 cell lysate.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated NFκB from A431 RIPA lysate.

Calidad

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NFκB in 10 µg of A431 cell lysate.

Descripción de destino

~65 kDa observed.
Four isoforms produced by alternative splicing have been described: Isoform 1 (p65) at 60.2 kDa, Isoform 2 (p65 delta 2) at 58.8 kDa, Isoform 3 (p65 delta) at 59.1 kDa, and Isoform 4 at 59.9 kDa

Ligadura / enlace

Replaces: 06-418

Forma física

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
A431 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable