861049

Azure A chloride

certified by the Biological Stain Commission, Dye content 70 %

• Sinónimos: Methylene blue
• Fórmula empírica (notación de Hill): C₁₄H₁₄ClN₃S
• Número de CAS: 531-53-3
• Peso molecular: 291.80
• Número de índice de color: 52005
• Beilstein: 3922287
• Número CE: 208-510-7
• Número MDL: MFCD00012112
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 329769437
• NACRES: NA.47

Propiedades

• grado: certified by the Biological Stain Commission
• Nivel de calidad: 200
• formulario: powder
• composición: Dye content, 70%
• color: blue to very dark blue; very dark green
• mp: 290 °C (dec.) (lit.)
• solubilidad: H₂O: 1 mg/mL
• λmáx.: 633 nm
• ε (coeficiente de extinción): ≥11000 at 242-246 nm in H₂O at -1; ≥26000 at 287-291 nm in H₂O; ≥34000 at 620-634 μm in H₂O
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]
• InChI: 1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
• Clave InChI: NALREUIWICQLPS-UHFFFAOYSA-N

Descripción

Aplicación

Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles.

Acciones bioquímicas o fisiológicas

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.

Idoneidad

Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)