T7699
Thunderbolt™ GC10™ Electrocompetent Cells
for generation of cDNA libraries and DNA plasmid production
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About This Item
MDL-Nummer:
UNSPSC-Code:
12352200
Empfohlene Produkte
Qualität
for molecular biology
Form
suspension
Versandbedingung
dry ice
Lagertemp.
−70°C
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Allgemeine Beschreibung
Thunderbolt GC10 electrocompetent E. coli have a very high transformation efficiency of >1x1010 cfu/μg when transformed with non-saturating amounts of pUC19 plasmid DNA. They are comparable to DH10β strain.
Anwendung
Suitable for recovery of high quality plasmid DNA and generation of cDNA libraries from plasmid-based vectors
Leistungsmerkmale und Vorteile
- Ensures recovery of stable, high quality plasmid DNA as well as methylated DNA
- Renders protection to clonal stocks from T1 and T5 bacteriophage contamination
- Allows for β-galactosidase α-complementation for blue/white screening
- Guaranteed high transformation efficiency
- Convenient 80 μL or 100 μL aliquots
Komponenten
- Thunderbolt GC10 electrocompetent cells, 5 X 80 μL or 5 X 100 μL (T7074)
- pUC 19 control DNA (10 ng/μL), 10 μL (D2567)
Prinzip
Thunderbolt GC10 electrocompetent E. coli is K strain bacteria that contain mutations in recA1 and endA1 genes. These mutations aid in minimizing recombination and ensuring plasmid stability. This strain also contains tonA genotype that confers resistance to lytic bacteriophages such as T1 and T5. The host restriction systems are eliminated to allow the cloning of methylated DNA.
Rechtliche Hinweise
GC10 is a trademark of GeneChoice, Inc.
Thunderbolt is a trademark of GeneChoice, Inc.
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Molecular ecology resources, 8(4), 742-752 (2008-07-01)
High throughput sequencing methods are widely used in analyses of microbial diversity, but are generally applied to small numbers of samples, which precludes characterization of patterns of microbial diversity across space and time. We have designed a primer-tagging approach that
Dana G Mordue et al.
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The apicomplexan parasite Toxoplasma gondii is able to suppress nitric oxide production in activated macrophages. A screen of over 6000 T. gondii insertional mutants identified two clones, which were consistently unable to suppress nitric oxide production from activated macrophages. One
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Journal of bacteriology, 190(20), 6779-6794 (2008-08-19)
The increasing occurrence of multidrug-resistant pathogens of clinical and agricultural importance is a global public health concern. While antimicrobial use in human and veterinary medicine is known to contribute to the dissemination of antimicrobial resistance, the impact of microbial communities
Plasmids of Escherichia coli as cloning vectors.
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