C4478
S-Gal®/LB Agar-Mischung
reagent for selection of recombinant bacterial clones
Synonym(e):
Agar Blend
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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About This Item
UNSPSC-Code:
41106200
NACRES:
NA.85
Empfohlene Produkte
Qualität
for molecular biology
Sterilität
non-sterile
Form
powder
Methode(n)
microbiological culture: suitable
Eignung
suitable for β-galactosidase test
nonselective for Escherichia coli
nonselective for coliforms
Anwendung(en)
food and beverages
microbiology
Lagertemp.
room temp
Verwandte Kategorien
Allgemeine Beschreibung
S-Gal® (sodium salt) is an autoclavable, water-soluble, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Anwendung
S-Gal®/LB Agar Blend has been used for the identification of recombinant bacterial colonies.
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® (sodium salt) is water-soluble, autoclavable and can be added to bacterial broth containing agar prior to autoclaving.
Leistungsmerkmale und Vorteile
- More intense color contrast than X-gal
- Water-soluble and autoclavable for easiest use
- Convenient, pre-mixed media
Komponenten
Zusammensetzung (g/L):
Trypton, 10
Hefeextrakt, 5
Natriumchlorid, 10
Agar, 12
S-Gal, 0.3
Eisen(III)-ammonium-citrat, 0.5
IPTG, 0.03
Trypton, 10
Hefeextrakt, 5
Natriumchlorid, 10
Agar, 12
S-Gal, 0.3
Eisen(III)-ammonium-citrat, 0.5
IPTG, 0.03
Prinzip
When S-Gal® is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.
Angaben zur Herstellung
Suspend contents of one packet in 500 ml distilled or deionized water. Sterilize by autoclaving for 15 to 20 minutes at 121-124°C. For microwaving, heat suspended mix until initial boiling. Mix well. Heat for short intervals with mixing until agar component is in solution. Do not allow boiling for extended periods of time. Antibiotics should be added following autoclaving or microwaving, after cooling to 48-52°C.
Sonstige Hinweise
The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
Rechtliche Hinweise
S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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K Heuermann et al.
BioTechniques, 30(5), 1142-1147 (2001-05-18)
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