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Wichtige Dokumente
L7658
LB-Bouillon
EZMix powder microbial growth medium
Synonym(e):
Lennox broth
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About This Item
UNSPSC-Code:
41106200
NACRES:
NA.85
Empfohlene Produkte
Qualität
for molecular biology
Qualitätsniveau
Beschreibung
quick-dissolve, dust-free formulation
Sterilität
non-sterile
Form
powder
Methode(n)
microbiological culture: suitable
pH-Wert
6.8-7.2(2.5% solution)
Anwendung(en)
agriculture
food and beverages
microbiology
Lagertemp.
room temp
Eignung
nonselective for Escherichia coli
nonselective for coliforms
Allgemeine Beschreibung
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation.
Anwendung
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Leistungsmerkmale und Vorteile
Lennox LB EZMix powder provides:
- Granulated, dust-free format for safer handling and faster mixing
- Convenient small package to eliminate weighing
- Easy scale-up using larger package sizes
- Standard formulation
Komponenten
*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
0.6 g/L inert binder (EZMix formulation only)
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
0.6 g/L inert binder (EZMix formulation only)
Angaben zur Herstellung
1. Suspend 20.6 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1M magnesium sulfate after autoclaving.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1M magnesium sulfate after autoclaving.
Rekonstituierung
Stir to suspend 20.6g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Rechtliche Hinweise
Zeocin is a registered trademark of Cayla Sarl
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Preisangaben
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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Protokolle
General protocols for growth of competent cells in microbial medium.
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