SRP0172

Jak2 Jh1Jh2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• Synonym(e): JAK2, JTK10, Janus kinase 2, Just another kinase 2, Tyrosine-protein kinase JAK2
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected insect cells
• Assay: ≥70% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 99 kDa
• Verpackung: pkg of 10 μg
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_004972
• UniProt-Hinterlegungsnummer: O60674
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... JAK2(3717)

Beschreibung

Allgemeine Beschreibung

Human Jak2 (GenBank Accession No. NM_004972), containing the catalytic and the pseudo kinase domain (amino acids 532-1132) with N-terminal GST-His tag, MW = 99 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling

Einheitendefinition

One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable