SRE0069

Phosphoglucomutase aus Kaninchenmuskel

• Synonym(e): αα-D-Glucose-1,6-bisphosphatase, αα-D-Glucose-1-phosphatphosphotransferase
• CAS-Nummer: 9001-81-4
• EC-Nummer: 5.4.2.2 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00131886
• UNSPSC-Code: 12352204

Eigenschaften

• Form: lyophilized powder
• Qualitätsniveau: 200
• Spezifische Aktivität: ≥100 units/mg protein
• Fremdaktivität: Lactic dehydroenase ≤0.5%; Phosphoglucose Isomerase ≤0.01%; pyruvate kinase ≤0.05%
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

The phosphoglucomutase (PGM) molecule, a single polypeptide chain, is made up of four α/β domains. The domains form a compact heart-shaped structure with a large fissure between the two lobes, and the molecule comprises about 40 secondary structural components. The active enzymatic site of the molecule is located at the bottom of the crevice on the surface of domain I. Rabbit muscle phosphoglucomutase (RB-PGM) is a monomer with unique four-domain architecture.

Anwendung

Phosphoglucomutase from rabbit muscle may be used in dephosphorylation to investigate the hexose bisphosphate activation of phosphoglucomutase.

Biochem./physiol. Wirkung

Phosphoglucomutase (PGM) mainly catalyzes the interconversion of glucose 1-phosphate and glucose 6-phosphate via a glucose 1,6-diphosphate intermediate. Hence it plays a key role in glycolysis and gluconeogenesis.

Einheitendefinition

One unit will convert 1.0 μmole of α-D-glucose 1-phosphate to α-D-glucose 6-phosphate per min at pH 7.4 at 30 °C.

Physikalische Form

Lyophilized powder containing Tris HCl, EDTA, magnesium acetate and carbohydrate

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3