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Merck

P4109

Sigma-Aldrich

β-Phosphoglucomutase from Lactococcus sp.

recombinant, expressed in E. coli

Synonym(e):

β-D-Glucose 1,6-phosphomutase

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About This Item

EC-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54

Rekombinant

expressed in E. coli

Qualitätsniveau

Form

solid

Spezifische Aktivität

≥10 unit/mg solid

Lagertemp.

−20°C

Verwandte Kategorien

Anwendung

β-Phosphoglucomutase is used to study starch and sucrose metabolism. It is used for the determination of α-amylase in clinical analysis. It is used to study galactose utilization and generalized glycogenosis (Pompe′s Disease).

Biochem./physiol. Wirkung

β-Phosphoglucomutase enzymatically converts β-D-glucose 1-phosphate to β-D-glucose 6-phosphate. It is involved in starch and sucrose metabolism.
Enzymatically converts β-D-glucose-1-phosphate to β-D-glucose-6-phosphate.

Einheitendefinition

One unit is defined as the amount of enzyme which converts 1μmol of β-D-glucose-1-phosphate to β-D-glucose-6-phosphate per minute at 37°C and pH 7.0.

Sonstige Hinweise

Contains EDTA and lactose.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Joanna L Griffin et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(18), 6910-6915 (2012-04-17)
Experimental observations of fluoromagnesate and fluoroaluminate complexes of β-phosphoglucomutase (β-PGM) have demonstrated the importance of charge balance in transition-state stabilization for phosphoryl transfer enzymes. Here, direct observations of ground-state analog complexes of β-PGM involving trifluoroberyllate establish that when the geometry
Jianying Dai et al.
Biochemistry, 45(25), 7818-7824 (2006-06-21)
Activated Lactococcus lactis beta-phosphoglucomutase (betaPGM) catalyzes the conversion of beta-d-glucose 1-phosphate (betaG1P) derived from maltose to beta-d-glucose 6-phosphate (G6P). Activation requires Mg(2+) binding and phosphorylation of the active site residue Asp8. Initial velocity techniques were used to define the steady-state
G G Hardy et al.
Infection and immunity, 69(4), 2309-2317 (2001-03-20)
Synthesis of the Streptococcus pneumoniae type 3 capsule requires the pathway glucose-6-phosphate (Glc-6-P) --> Glc-1-P --> UDP-Glc --> UDP-glucuronic acid (UDP-GlcUA) --> (GlcUA-Glc)(n). The UDP-Glc dehydrogenase and synthase necessary for the latter two steps, and essential for capsule production, are
OCULAR HISTOPATHOLOGY IN GENERALIZED GLYCOGENOSIS (POMEPE'S DISEASE).
D TOUSSAINT et al.
Archives of ophthalmology (Chicago, Ill. : 1960), 73, 342-349 (1965-03-01)
A phosphoglucomutase specific for β-glucose 1-phosphate
Ben-Zvi R and Schramm M
The Journal of Biological Chemistry, 236, 2186-2189 (1961)

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