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Merck

L9267

Sigma-Aldrich

Lectin from Lens culinaris (lentil)

lyophilized powder

Synonym(e):

Lens culinaris agglutinin, LcH

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About This Item

UNSPSC-Code:
12352202
NACRES:
NA.32

Form

lyophilized powder

Qualitätsniveau

Wirksamkeit

<16 μg per mL agglutination activity

Verunreinigungen

salt, free

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.

Anwendung

Lectin from Lens culinaris (lentil) is used for size- or charge-based separation of proteins with the ANA. (Anisotropic Nanofilter Array: ANA).

Biochem./physiol. Wirkung

LcH is not blood group specific. It has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. LcH is comprised of two isomers, LcH-A (mol. wt. 49,000) and LcH-B (mol. wt. 49,000). LcH-A is mitogenic. Conjugates are prepared from purified lectin.

Qualität

Contains two major bands on electrophoresis corresponding to isolectins LcH-A and LcH-B.

Angaben zur Herstellung

Highly purified by affinity chromatography.

Hinweis zur Analyse

Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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