L8275

Lectin from Galanthus nivalis (snowdrop)

lyophilized powder

• Synonym(e): Galanthus nivalis lectin, GNL
• MDL-Nummer: MFCD00163718
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Form: lyophilized powder
• Qualitätsniveau: 200
• Wirksamkeit: <20 μg per mL agglutination activity
• Zusammensetzung: Protein, ≥90% Lowry
• Verunreinigungen: salt, essentially free
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Lectin from Galanthus nivalis (snowdrop) is a tetrameric, 12 kDa mannose binding lectin. It belongs to the family, Amaryllidacea. It is specific to α-1,3- or 1,6- linked D-mannose of carbohydrates. Snowdrop lectins are toxic to insects.

Anwendung

Lectin from Galanthus nivalis (snowdrop) has been used:

• to coat ELISA plates
• as reverse transcriptase inhibitor
• in ELISA for detection of envelope glycoproteins of the human and simian immunodeficiency viruses

Biochem./physiol. Wirkung

Lectin from Galanthus nivalis (snowdrop) provide resistance against pests and are used in transgenic plants. Snowdrop lectin functions as a carrier to transport fused proteins to the hemolymph as it crosses gut epithelium of target species to exhibit insecticidal effects.

GNL agglutinates rabbit erythrocytes; human erythrocytes are not agglutinated. GNL sugar specificity is directed to the nonreducing end of the terminal α-D-mannosyl residue of glycoconjugates.

Angaben zur Herstellung

Purified by affinity chromatography according to Kaku.

Hinweis zur Analyse

Agglutination activity is expressed in μg/ml and is determined from serial dilution in phosphate buffered saline, pH 7.2 of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of rabbit erythrocytes after 1 hr incubation at 25 °C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)