GE17-5279-02
Glutathione Seph High Performance
Cytiva 17-5279-02, pack of 100 mL
About This Item
Empfohlene Produkte
ligand
glutathione
Verpackung
pack of 100 mL
Hersteller/Markenname
Cytiva 17-5279-02
Matrix
highly cross-linked 6% agarose
Durchschnittliche Teilgröße
34 μm
cleaning
3-12
Arbeitsbereich
3-12
Kapazität
>7 mg/mL binding capacity(medium)
Verwandte Kategorien
Allgemeine Beschreibung
Anwendung
Leistungsmerkmale und Vorteile
- 34 μm bead size for improved efficiency and resolution
- Fast, one-step purification of GST-tagged Proteins and other glutathione S-trasferase or glutathione-dependent Proteins
- Mild conditions during the purification process preserves Protein antigenicity and function
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
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Analysenzertifikate (COA)
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Artikel
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
Protokolle
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap columns packed with Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, or Glutathione Sepharose® 4B chromatography media.
Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.
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