E5529
EZview™ Red Streptavidin Affinity Gel
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About This Item
UNSPSC-Code:
41106500
NACRES:
NA.56
Empfohlene Produkte
Form
suspension
Haltbarkeit
1 yr
Methode(n)
western blot: suitable
Matrix
4% agarose
pH-Wert
7.2
Kapazität
~10 μg(biotin per ml of packed gel)
Versandbedingung
wet ice
Lagertemp.
2-8°C
Allgemeine Beschreibung
The EZ View Red Streptavidin Affinity gel is composed of streptavidin, covalently attached to
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.
cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands.
Anwendung
Sutitable for use with immunoprecipitation, western blotting,and enzyme assays.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Leistungsmerkmale und Vorteile
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Physikalische Form
1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
Rechtliche Hinweise
EZview is a trademark of Sigma-Aldrich Co. LLC
Lagerklassenschlüssel
10 - Combustible liquids
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Verwandter Inhalt
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
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