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GE17-5279-01

Glutathione Sepharose High Performance

Cytiva 17-5279-01, pack of 25 mL

Synonym(e):

Glutathione Sepharose Column

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About This Item

UNSPSC-Code:
41106500
NACRES:
NA.56

ligand

glutathione

Verpackung

pack of 25 mL

Hersteller/Markenname

Cytiva 17-5279-01

Matrix

highly cross-linked 6% agarose

Durchschnittliche Teilgröße

34 μm

cleaning

3-12

Arbeitsbereich

3-12

Kapazität

>7 mg/mL binding capacity(medium)

Allgemeine Beschreibung

Glutathione Sepharose High Performance is an affinity medium for high resolution purification of Glutathione S transferase (GST) tagged proteins.

Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function.

Leistungsmerkmale und Vorteile

  • 34 μm bead size for improved efficiency and resolution
  • Fast, one-step purification of GST-tagged proteins and other glutathione S-trasferase or glutathione-dependent proteins
  • Mild conditions during the purification process preserves protein antigenicity and function

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Sepharose is a trademark of Cytiva

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Lagerklassenschlüssel

3 - Flammable liquids


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Artikel

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

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Protokolle

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap columns packed with Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, or Glutathione Sepharose® 4B chromatography media.

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