GE17-5279-01
Glutathione Sepharose™ High Performance
Cytiva 17-5279-01, pack of 25 mL
Synonym(e):
Glutathione Sepharose Column
About This Item
Empfohlene Produkte
ligand
glutathione
Verpackung
pack of 25 mL
Hersteller/Markenname
Cytiva 17-5279-01
Matrix
highly cross-linked 6% agarose
Durchschnittliche Teilgröße
34 μm
cleaning
3-12
Arbeitsbereich
3-12
Kapazität
>7 mg/mL binding capacity(medium)
Allgemeine Beschreibung
Glutathione Sepharose™ High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function.
Leistungsmerkmale und Vorteile
- 34 μm bead size for improved efficiency and resolution
- Fast, one-step purification of GST-tagged proteins and other glutathione S-trasferase or glutathione-dependent proteins
- Mild conditions during the purification process preserves protein antigenicity and function
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Hier finden Sie alle aktuellen Versionen:
Analysenzertifikate (COA)
Leider sind derzeit keine COAs für dieses Produkt online verfügbar.
Wenn Sie Hilfe benötigen, wenden Sie sich bitte an Kundensupport
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Artikel
This page describes general considerations for purification of GST-tagged proteins using Cytiva products.
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
Protokolle
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap columns packed with Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, or Glutathione Sepharose® 4B chromatography media.
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.