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G6008
ββ-Galactosidase aus E. coli
Grade VI, lyophilized powder, ≥250 units/mg protein
Synonym(e):
β-D-Galactosid-Galactohydrolase, Lactase
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About This Item
Empfohlene Produkte
Biologische Quelle
Escherichia coli
Qualitätsniveau
Typ
Grade VI
Form
lyophilized powder
Spezifische Aktivität
≥250 units/mg protein
Mol-Gew.
465 kDa
Enthält nicht
BSA as extender
Zusammensetzung
Protein, ≥50% biuret
Versandbedingung
wet ice
Lagertemp.
−20°C
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Allgemeine Beschreibung
β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 Da each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.
Anwendung
β-Galactosidase is conjugated to an antibody that specifically recognizes a target molecule (enzyme immunoassay or EIA). β-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter.
Biochem./physiol. Wirkung
β-Galactosidase spaltet Lactose in ihre Monosaccharid-Komponenten Glucose und Galactose. Außerdem katalysiert sie die Transglycosylierung von Glucose zu Allolactose, dem Induktor von β-Galactosidase, in einem Rückführungsmechanismus.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Physikalische Eigenschaften
Molekulargewicht des Tetramers: 465 kDa (Subunits je 116,3 kDa)
Einheitendefinition
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Physikalische Form
Partially purified; contains Tris buffer salts, magnesium chloride, DL-dithiothreitol and 2-mercaptoethanol
Inhibitor
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Substrat
Produkt-Nr.
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Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.
G R CRAVEN et al.
The Journal of biological chemistry, 240, 2468-2477 (1965-06-01)
D C Young et al.
Analytical biochemistry, 215(1), 24-30 (1993-11-15)
Bacterial beta-galactosidase is one of several reporter enzymes used in studying the transcriptional activity of eukaryotic promoters. Although it is one of the easiest and least expensive enzymes to assay, its use has been limited because of its low sensitivity
K Kato et al.
Journal of immunology (Baltimore, Md. : 1950), 116(6), 1554-1560 (1976-06-01)
1. A method for the conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from Escherichia coli is described. The method consists of two main steps: treatment of the Fab' fragments containing sulfhydryl groups with excess N,N'-o-phenylenedimaleimide, to introduce
D H Juers et al.
Protein science : a publication of the Protein Society, 8(1), 122-136 (1999-04-21)
Beta-galactosidase (lacZ) from Escherichia coli is a 464 kDa homotetramer. Each subunit consists of five domains, the third being an alpha/beta barrel that contains most of the active site residues. A comparison is made between each of the domains and
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