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Merck

F9137

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(e):

Rabbit Anti-Mouse IgG (whole molecule)−Fluorescein isothiocyanate

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

rabbit

Konjugat

FITC conjugate

Antikörperform

IgG fraction of antiserum

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Lagerbedingungen

protect from light

Methode(n)

direct immunofluorescence: 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

IgG antibodies are the smallest immunoglobulins found in all body fluids and can fight against bacterial and viral infections. It is the only type of antibody that can cross the placenta in pregnant woman and give immunity to the foetus. Anti-mouse IgG (whole molecule)-FITC antibody can be used as a secondary antibody in FACS analysis to detect cell surface expression of cd59b and cd59a in cloned CHO cells. Rabbit anti-mouse IgG (whole molecule)-FITC antibody reacts specifically with mouse IgG, IgA and IgM.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (whole molecule)-FITC antibody reacts with all mouse IgG subclasses and IgM. Purified rabbit anti-Mouse IgG is conjugated to FITC in an alkaline reaction.

Spezifität

Specificity for mouse IgG is determined by immunoelectrophoresis (IEP) prior to conjugation using normal mouse serum and mouse IgG. Product reacts with mouse IgA and IgM.

Immunogen

IgG from pooled normal mouse serum

Anwendung

Anti-Mouse IgG (whole molecule)-FITC antibody may be used for immunofluorescence at a working antibody dilution of 1:100 on human tonsil sections using mouse monoclonal anti-human IgG as primary antibody. For immunofluorescence of human peripheral blood lymphocytes, a minimum dilution of 1:100 is recommended. For immunohistochemistry applications, the working antibody dilutions range from 1:100 to 1:1000 in various studies.
Anti-mouse IgG (whole molecule)-FITC antibody (diluted 1:1000) can be used as secondary antibody in immunohistochemistry.
Anti-Mouse IgG (whole molecule)-FITC antibody has been used in immunofluorescence and cell proliferation assay.

Biochem./physiol. Wirkung

Immunoglobulin G (IgG) mainly helps in immune defense. It participates in hypersensitivity type II and type III. It also helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Lagerung und Haltbarkeit

Store at 2-8 °C for a maximum of one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Calabi F and Neuberger MS
Molecular Genetics of Immunoglobulin (1987)
Lack of experience-mediated differences in the immunohistochemical expression of blood-brain barrier markers (EBA and GluT-1) during the postnatal development of the rat visual cortex
Argando?a EG et al
Brain Research. Brain Research Reviews, 156, 158-166 (2005)
Regulation of Claspin degradation by the ubiquitin-proteosome pathway during the cell cycle and in response to ATR-dependent checkpoint activation.
Bennett LN and Clarke PR
Febs Letters, 580(17), 4176-4181 (2002)
Miguel Gonzalez-Andrades et al.
Investigative ophthalmology & visual science, 52(1), 215-222 (2010-08-27)
Decellularization of animal corneas is a promising method for the development of artificial human corneas by tissue engineering. In this study, two different decellularization protocols were evaluated to determine which one is able to best preserve the histologic structure, composition
Polychlorinated biphenyls (PCBs) alter DNA methylation and genomic integrity of sheep fetal cells in a simplified in vitro model of pregnancy exposure.
Anzalone DA, et al.
Toxicology in vitro, 46, 39-46 (2018)

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