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Merck

C2181

Sigma-Aldrich

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Cy3 antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Cy3 Anti-Mouse IgG, Cy3 Mouse IgG, Sheep Anti-Mouse IgG

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

sheep

Konjugat

CY3 conjugate

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Speziesreaktivität

mouse

Methode(n)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

Versandbedingung

wet ice

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4.. The IgG heavy chain region is mapped to human chromosome 14. Igs have two heavy (H) and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region.

Anwendung

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-Cy3 antibody produced in sheep was used for BrdU staining of frozen quail muscle cross sections at a dilution of 1:200 to analyze activated, proliferating muscle precursor cells.
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment−Cy3 antibody produced in sheep has been used:
  • in immunolabeling of Hela cells
  • as secondary antibody in immunocytochemistry of dendritic cells
  • as secondary antibody in immunofluorescence analysis of mesenchymal stem cells
  • as secondary antibody in immunofluorescence staining keratinocyte cell lines
  • in immunohistochemistry of articular cartilage

The product binds to all mouse Igs and is useful when trying to avoid background staining due to the presence of Fc receptors.

Biochem./physiol. Wirkung

Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Mouse IgG (whole molecule) F(ab′)2 fragment antibody allows for the visualization of protein by fluorescent microscopy.

Sonstige Hinweise

Antibody adsorbed with human serum proteins.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Angaben zur Herstellung

Adsorbed to reduce background staining with human samples.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Identification of a Novel Keratin 9 Missense Mutation in a Chinese Family with Epidermolytic Palmoplantar Keratoderma.
Xiao H, et al.
Cellular Physiology and Biochemistry, 46(5), 1919-1929 (2018)
A Birkmann et al.
Journal of virology, 75(23), 11583-11593 (2001-11-02)
An immunodominant envelope glycoprotein is encoded by the human herpesvirus 8 (HHV-8) (also termed Kaposi's sarcoma-associated herpesvirus) K8.1 gene. The functional role of glycoprotein K8.1 is unknown, and recognizable sequence homology to K8.1 is not detectable in the genomes of
Agili-C implant promotes the regenerative capacity of articular cartilage defects in an ex vivo model.
Chubinskaya S, et al.
Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA, 46(5), 1-12 (2018)
Antonio F Ribeiro et al.
Scientific reports, 9(1), 11842-11842 (2019-08-16)
Satellite cells (SCs) are the main muscle stem cells responsible for its regenerative capacity. In muscular dystrophies, however, a failure of the regenerative process results in muscle degeneration and weakness. To analyze the effect of different degrees of muscle degeneration
Visualization of altered replication dynamics after DNA damage in human cells.
Merrick C, et al.
The Journal of Biological Chemistry, 279(19), 20067-20075 (2004)

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