D9944

Monoclonal Anti-DYNLT1 antibody produced in mouse

~1.0 mg/mL, clone T1, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Anti-CW-1, Anti-Dynein, light chain, Tctex-type 1, Anti-TCTEL1, Anti-Tctex-1
• UNSPSC-Code: 12352203
• NACRES: NA.41
• Klon: T1, monoclonal
• application: ICC; WB
• Speziesreaktivität: monkey, mouse, bovine, human, rat
• Methode(n): immunocytochemistry: suitable; western blot: 8-16 μg/mL using NRK total cell lysate
• citations: 9

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: T1, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~12.5 kDa
• Speziesreaktivität: monkey, mouse, bovine, human, rat
• Konzentration: ~1.0 mg/mL
• Methode(n): immunocytochemistry: suitable; western blot: 8-16 μg/mL using NRK total cell lysate
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P63172
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... DYNLT1(6993) ; mouse ... Dynlt1(21648) ; rat ... Dynlt1(83462)

Beschreibung

Immunogen

recombinant dynein 1 light chain-myosin basic protein fusion protein.

Anwendung

Monoclonal Anti-DYNLT1 antibody produced in mouse is suitable for immunocytochemistry and immunoblotting at a working concentration of 8-16μg/mL using NRK total cell lysate.

Biochem./physiol. Wirkung

Cytoplasmic dynein motor complex is responsible for minus-end, microtubule-based motile processes. It is thus involved in a wide range of eukaryotic cellular processes including trafficking of membranal vesicles, viruses and other intracellular particles. DYNLT1 is a light chain subunit of the complex that is involved in cargo binding. It binds specifically to the cytoplasmic tail of rhodopsin and links rhodopsin-bearing vesicles to the dynein motor complex for transport. The cargo is released by the disassembly of dynein complex upon phosphorylation of DYNLT1 on Ser⁸² residue. This process is critical for the apical delivery of membrane cargoes. It also mediates neurite extension by modulating actin dynamics and also Rac1 activity. The abundance and expression pattern of DYNLT1 in neuronal tissues may also contribute to the invasion capabilities of neurotropic viruses like poliovirus.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)