D8940

Anti-Destrin/ADF (AE-14) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-Actin Depolymerizing Factor
• MDL-Nummer: MFCD03791499
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Speziesreaktivität: mouse, rat, human
• Konzentration: ~0.8-1.2 mg/mL
• Methode(n): microarray: suitable; western blot: 1:600 using whole extracts of human A431 epidermoid carcinoma cells, rat PC-12 pheochromocytoma cells, or mouse NIH3T3 fibroblasts
• UniProt-Hinterlegungsnummer: P60981
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... DSTN(11034) ; mouse ... Dstn(56431) ; rat ... LOC296197(296197)

Beschreibung

Allgemeine Beschreibung

Destrin/ADF and cofilin are widely distributed in tissues of eukaryotes and both contain a nuclear localization sequence. It is found in various epithelial and endothelial cells but is practically absent from adult mouse heart and skeletal muscle cells. This gene is located on human chromosome 20p12.

Destrin/ADF is usually found in regions containing dynamic actin pools such as the leading edge of migrating cells and neuronal growth cones and may also colocalize in cell nuclei. It is also present in ‘Hirano bodies′ in certain brain neurons of dementia patients.

Immunogen

synthetic peptide corresponding to amino acids 137-150 of human destrin/ADF.

Anwendung

Anti-Destrin/ADF antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:600 using whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and mouse NIH/3T3 fibroblasts, and a chemiluminescent detection reagent.

Biochem./physiol. Wirkung

Destrin/ADF (Actin Depolymerizing Factor) is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in a pH-dependent, Ca²⁺ independent manner. It intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. It is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, and cytokinesis.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable