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A2522

Sigma-Aldrich

Aktin aus Kaninchenmuskel

≥85% (SDS-PAGE), lyophilized powder

Synonym(e):

Alpha-actin 1, G-Actin

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About This Item

CAS-Nummer:
51005-14-2
MDL-Nummer:
MFCD00130338
UNSPSC-Code:
12352202
NACRES:
NA.32

Biologische Quelle

rabbit

Qualitätsniveau

200

Assay

≥85% (SDS-PAGE)

Form

lyophilized powder

Mol-Gew.

43 kDa

Zusammensetzung

Protein, ≥80% biuret

Methode(n)

immunoblotting: suitable

UniProt-Hinterlegungsnummer

P68135

Lagertemp.

2-8°C

Angaben zum Gen

rabbit ... LOC(100342017)

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Allgemeine Beschreibung

Actin is the abundant protein found in many cells which accounts for 10% or more of total protein. Most of the eukaryotic cells shows the presence of actin protein. Outer and inner domains are the two important domains of actin.

Anwendung

Actin from rabbit muscle has been used:
  • to study its protective role on rabbit muscle lactate dehydrogenase from ascorbate inhibition
  • as standard in SDS gel and immunoblotting
  • in the protein pin array assays

Biochem./physiol. Wirkung

Actin plays a key role in protein-protein interactions. It participates in cell motility, cell shape maintenance and polarity to the regulation of transcription.

Physikalische Form

Lyophilized powder containing Tris, ATP and CaCl2

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Actin and Actin-Binding Proteins
Pollard TD, et al.
Cold Spring Harbor Perspectives in Biology, 8(8) (2016)
Desmin cytoskeletal modifications after a bout of eccentric exercise in the rat
Barash IA, et al.
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 91-98 (2002)
Pooneh Vaziri et al.
Genetics, 216(1), 177-190 (2020-08-06)
Myosin is an essential motor protein, which in muscle is comprised of two molecules each of myosin heavy-chain (MHC), the essential or alkali myosin light-chain 1 (MLC1), and the regulatory myosin light-chain 2 (MLC2). It has been shown previously that MLC2
Interactive sequences in the stress protein and molecular chaperone human alphaB crystallin recognize and modulate the assembly of filaments
Ghosh JG, et al.
The International Journal of Biochemistry & Cell Biology, 39(10), 1804-1815 (2007)
J A Henderson et al.
Endocrine, 33(3), 285-293 (2008-11-21)
To further understand the role of ovarian hormones in the function of the serotonin neural system, we investigated the effects of estradiol (E), progesterone (P), and raloxifene on 5HT 1A and 2C receptor protein expression in the dorsal raphe region
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Artikel

Actin

Although actin is one of the most conserved eukaryotic proteins, it is expressed as six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of the six isoforms represent differentiation markers of muscle tissues. The other two are found in practically all cells.

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