EMT6/MTXR

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: mouse unknown/unspecified
• Verpackung: tube of 5 μg 96042322-DNA-5UG; pkg of vial of cells 96042322-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse mammary, drug-resistant

Beschreibung der Zelllinie

The drug-resistant cell line EMT6/MTXR was derived from the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344) by growth in increasing concentrations of methotrexate. EMT6/MTXR did not hyperexpress P-glycoprotein and did not show cross-resistance to other drugs of the multi-drug resistance (MDR) phenotype. It is recommended to culture the cells without drug on resuscitation until first passage or growth has been fully established.

Anwendung

Drug-resistance studies

Nährmedium

MEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1μg/ml methotrexate + 15% Foetal Bovine Serum (FBS).. Resuscitate in drug-free medium; add drug once growth is fully established

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA;5% CO₂; 37ºC

Sonstige Hinweise

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