PC-9 (formerly known as PC-14)

90071810, human lung, Heterogeneous mixture of round cells and spindle shaped cells

• Synonym(e): PC-9/S1 Cells, PC-9S1 Cells, PC9 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• product name: PC-9 (formerly known as PC-14), 90071810
• Biologische Quelle: human lung
• Wachstumsmodus: Adherent/suspension
• Karyotyp: Not specified
• Morphologie: Heterogeneous mixture of round cells and spindle shaped cells
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human adenocarcinoma

Beschreibung der Zelllinie

Derived from a human adenocarcinoma from lung tissue which remains undifferentiated.

PC-14 was originally deposited with the Riken BioResource Center as a cell line derived from human lung adenocarcinoma (undifferentiated type) in 1989. The RIKEN BioResource Center subsequently informed the European Collection of Cell Cultures (ECACC) that short tandem repeat (STR) DNA profile analysis, undertaken in collaboration Immuno-Biological Laboratories Co., Ltd Japan, has shown this cell line to be identical to PC-9 a cell line derived from human lung adenocarcinoma (differentiated type). The misidentification occurred prior to the cell line being deposited at the RIKEN BioResource Center. The name of the cell line has been changed to PC-9 to reflect this finding.

Anwendung

PC-9 cell line has been used to study the effects of α-tocopherol addition on various cytotoxic drugs.

Tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8
D16S539: 9
D5S818: 11
D7S820: 10
THO1: 7
TPOX: 11
vWA: 17

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Seed cells at 2-4 x 10,000 cells/cm²; 5% CO₂; 37°C. Remove attached cells by brief treatment with 0.25% trypsin or trypsin/EDTA.  

Sonstige Hinweise

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