HR5-CL11

95091411

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human cervix
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human cervix carcinoma, HeLa derivative, transformed

Beschreibung der Zelllinie

HR5-CL11 cells were obtained after transfection of HeLa cells with plasmids coding for a transcriptional transactivator and transferring G418 and hygromycin resistance. This tetracycline-controlled transactivator (a fusion between the tet repressor and the activating domain of the herpes simplex virus VP16 transcription activation domain) can stimulate the expression of a reporter gene up to 100,000 fold. Subsequently these cells were stably transfected with a luciferase reporter unit. Luciferase expression in the HR5-CL11 cells is highly stimulated by doxycycline.

Anwendung

Gene expression and activation studies

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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