HT115

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85061104, human colon, Epithelial

• Synonym(e): HT-115 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human colon
• Verpackung: tube of 5 μg 85061104-DNA-5UG; pkg of vial of cells 85061104-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human colon carcinoma

Beschreibung der Zelllinie

Cells possess Ca antigen as defined by the Ca1 antibody. The Ca antigen has been shown to be absent in non-malignant human cell strains and is considered to be a marker linked with malignancy. Island cell growth will degenerate if left more than 9 days between passages.

Anwendung

Tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 7,12
D13S317: 13
D16S539: 10
D5S818: 12
D7S820: 11,12
THO1: 6,9
TPOX: 8
vWA: 18

Nährmedium

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells grow relatively slowly, in islands.

Sonstige Hinweise

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