BRL-1

94121649, rat liver, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: BRL-1, 94121649
• Biologische Quelle: rat liver
• Wachstumsmodus: Adherent
• Karyotyp: Diploid or pseudodiploid
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat epithelial cells

Beschreibung der Zelllinie

Isolated by Dr Hayden Coon. Flat epithelial cell colonies that appear upon low density plating as suspensions of collagenase dissociated buffalo rat liver. Cell origin not clearly defined.

Anwendung

Feeder cells for co-culture with freshly isolated hepatocytes.

Nährmedium

Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Subkultur-Routine

Spit confluent cultures 1:100 i.e. seeding at 1x1,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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