H4II

89042702, rat skin, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: H4II, 89042702
• Biologische Quelle: rat skin
• Wachstumsmodus: Adherent
• Karyotyp: 52 chromosomes
• Morphologie: Epithelial
• Produkte: Albumin, transferrin, prothrombin
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat skin Hepatocellular carcinoma

Beschreibung der Zelllinie

From H-35 Reuber hepatoma induced by chemical carcinogenesis in an AxC rat . Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin and prothrombin. (Same as H4-11-E-C3 (Sigma Catalogue number. 85061112)).

Anwendung

Gene expression analysis.

Nährmedium

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split semi-confluent [60%-70%] cultures 1:100 i.e. seeding at 2x100 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1000,000 cells/10cm² dish.

Sonstige Hinweise

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