BW1J

94101901, mouse skin, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: BW1J, 94101901
• Biologische Quelle: mouse skin
• Verpackung: tube of 5 μg 94101901-DNA-5UG; pkg of vial of cells 94101901-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Hypotetraploid
• Morphologie: Epithelial
• Produkte: Alpha-foetoprotein and Albumin
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse hepatoma

Beschreibung der Zelllinie

A mouse hepatoma isolated from the BW7756 transplantable hepatoma carried in C57 leaden/J mice (see reference: Differentiation). Will synthesise in appreciable amounts only hepatic proteins that appear early in foetal development. The majority of cells contain at least two and often several nucleoli, fat droplets are rare. A mixture of BW1-J and Fao cells were treated with inactive Sendai Virus to produce hybrids at 1 hybrid colony/50 BW1-J cells.

Nährmedium

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS)

Subkultur-Routine

Split semi-confluent cultures (70%) 1:50 i.e. seeding at 2x1000 - 5x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change three times a week.

Sonstige Hinweise

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