FGC4

94101904, rat skin, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: FGC4, 94101904
• Biologische Quelle: rat skin
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 52
• Morphologie: Epithelial
• Produkte: Albumin and ß-fibrogen
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat hepatoma [alb-gpt plasmid]

Beschreibung der Zelllinie

A subclone derived from Fao which is deficient in HGPRT (resistant to 5μg/ml 6-thioguanine and also resistant to 3mM ouabain). FGC4 contains the alb-gpt plasmid which is the bacterial gpt gene under control of the rat albumin promoter (400 bp). Produces albumin and ß-fibrinogen.GMO1

Anwendung

Gene expression studies.

Nährmedium

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split semi-confluent cultures (70%) 1:100 i.e. seeding at 2x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change three times a week.

Sonstige Hinweise

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