U-2 OS Cell Line human

92022711, osteosarcoma

• Synonym(e): U-2OS Cells, U2-OS Cells, U20S Cells, U2OS Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human bone
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Osteosarcoma

Beschreibung der Zelllinie

Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.

Anwendung

U-2 OS has been used to study:

• the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
• the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
• calcium-mediated actin reset (CaAR) in response to physiological changes

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18

Nährmedium

McCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.

Sonstige Hinweise

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