KELLY Cell Line human

92110411, neuroblastoma

• Synonym(e): NB19 Cells, NB19-RIKEN Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human brain
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Neuronal
• Produkte: N-myc RNA or protein
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human neuroblastoma

Beschreibung der Zelllinie

The cells possess a genomic amplification of the N-myc gene and express elevated levels of N-myc RNA or protein.

Anwendung

KELLY cell line has been used to study the role of polysialic acid (PSA) in controlling tumor cell growth and differentiation by interfering with neural cell adhesion molecule (NCAM) signaling. It has also been used to study the role of protein tyrosine phosphatase SHP1 in inhibiting Ret oncogene activity.

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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