SK.N.SH Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012802, human nerve (bone marrow metastasis; Caucasian neuroblastoma), Epithelial

• Synonym(e): NSH Cells, SK-NSH Cells, SKNSH Cells
• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human nerve (bone marrow metastasis; Caucasian neuroblastoma)
• Verpackung: tube of 5 μg 86012802-DNA-5UG; pkg of vial of cells 86012802-1VL
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 46, modal no. 47
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): metastasis
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian neuroblastoma

Beschreibung der Zelllinie

Established from a bone marrow metastasis from a 4-year-old caucasian female suffering with neuroblastoma. The cells have been used as a target cell in cell-mediated cytoxicity tests.

Anwendung

Human SK.N.SH cell line has been used in dark toxicity testing of a chloro-indium-phthalocyanine photosensitizer (In-Pc). It has also been used to study the potential mechanisms and domain requirements of the hHSJ1a (DnaJ heat shock protein family member B2) mediated reduction in superoxide dismutase 1 (SOD1) aggregation.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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