NCTC clone 1469

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88111403, mouse liver, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: mouse liver
• Verpackung: tube of 5 μg 88111403-DNA-5UG; pkg of vial of cells 88111403-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse C3H/An liver

Beschreibung der Zelllinie

Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).

Anwendung

Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)

Nährmedium

NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO₂; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan

Sonstige Hinweise

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