AB12 cell line from mouse lung

10092306, mouse lung, Cells are stellate-shaped

• UNSPSC-Code: 41106514

Eigenschaften

• product name: AB12 cell line from mouse lung, 10092306
• Biologische Quelle: mouse lung
• Beschreibung: Malignant mesothelioma from mouse lung
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Cells are stellate-shaped
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse malignant mesothelioma

Beschreibung der Zelllinie

Mice (BALB/c female 6-8 weeks) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0146

Anwendung

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Nährmedium

RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 5% FCS

Subkultur-Routine

Optimal split ratio 1:16 - 1:32 (seeding density 0.8 x104 cells/cm²). Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Sonstige Hinweise

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake - University of Western Australia, Australia