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Merck

C7512

Sigma-Aldrich

Butyrylcholinesterase aus Pferdeserum

lyophilized powder, ≥10 units/mg protein

Synonym(e):

Acylcholin-Acyl-hydrolase, Cholin-Esterase, Butyryl, Pseudocholinsterase

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About This Item

CAS-Nummer:
EC-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54

Form

lyophilized powder

Qualitätsniveau

Spezifische Aktivität

≥10 units/mg protein

Mol-Gew.

tetramer 440 kDa

Zusammensetzung

Protein, ≥60%

Löslichkeit

cold water: 60 U/mL

Lagertemp.

−20°C

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Allgemeine Beschreibung

Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). It is also termed as pseudocholinesterase. It is produced in the liver. It is found in blood, the synapse of neuromuscular junctions and glia cells and axons of white matter.

Anwendung

Butyrylcholinesterase from equine serum has been used in a microcalorimetric study of the inhibition of butyrylcholinesterase by paraoxon. This enzyme has also been used in a study to investigate the synthesis and inhibition of cholinergic enzymes. Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity has shown clinical potential, as there is a correlation between the blood level of BChE in humans and the degree of protection against toxic nerve agents. There has also been an interest in the roles of cholinesterases with regard to Alzheimer′s disease. Investigations into selective inhibitors may provide a clearer picture of the physiological role of BChE in both healthy and diseased individuals. This product has been used for the screening of cholineesterase inhibitors in selected fruits and vegetables, for restoring cognitive function and improving memory. It has also been used to develop a butyrylcholinesterase and choline oxidase immobilized bio-sniffer for the detection of nicotine. Nicotine inhibits BChE activity. A decrease in the byproducts of BChE activity reflects the volume of nicotine.

Biochem./physiol. Wirkung

Butyrylcholinesterase (BChE) can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is activated by Ca2+ and Mg2+ and the activity is constant over the pH range 6.0-8.0. It is inhibited by betaine, nicotine, organophosphates, and carbamates.
Mutations in butyrylcholinesterase (BChE) gene results in the failure of respiratory system, prolonged paralysis and apnea.

Einheitendefinition

One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.

Physikalische Form

Lyophilized powder containing buffer salts

Angaben zur Herstellung

This product is prepared from equine serum using ammonium sulfate fractionation and supplied as a lyophilized powder.

Hinweis zur Analyse

Protein determined by biuret

Inhibitor

Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Screening for cholinesterase inhibitors in selected fruits and vegetables.
Szwajgier D and Borowiec K
Biotechnology, 15(2) (2012)
Toxicokinetics of Chemical Warfare Agents: Nerve Agents and Vesicants
Handbook of Toxicology of Chemical Warfare Agents, 755-790 (2009)
Population-scale genomics-Enabling precision public health
Advances in Genetics, 103, 119-161 (2019)
Determinants of intracellular pH in the erythrocyte.
J Warth et al.
British journal of haematology, 29(3), 369-372 (1975-03-01)
Siti Munirah Mohd Faudzi et al.
Archiv der Pharmazie, 354(1), e2000161-e2000161 (2020-09-05)
A new series of pyrazole, phenylpyrazole, and pyrazoline analogs of diarylpentanoids (excluding compounds 3a, 4a, 5a, and 5b) was pan-assay interference compounds-filtered and synthesized via the reaction of diarylpentanoids with hydrazine monohydrate and phenylhydrazine. Each analog was evaluated for its

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