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C4018

Sigma-Aldrich

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

lyophilized powder, ≥100 units/mg protein

Synonym(e):

3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase

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About This Item

CAS-Nummer:
37289-07-9
EC-Nummer:
3.5.1.24 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00130789
UNSPSC-Code:
12352204
NACRES:
NA.54

Form

lyophilized powder

Spezifische Aktivität

≥100 units/mg protein

Zusammensetzung

Protein, ≥50% biuret

Versandbedingung

wet ice

Lagertemp.

−20°C

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Anwendung

The enzyme from Sigma has been used in the analysis of bile samples in various studies.

Biochem./physiol. Wirkung

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.

Einheitendefinition

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

Physikalische Form

Partially purified lyophilized powder containing buffer salts and stabilizer

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

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Bile salt hydrolase activity in probiotics.
Máire Begley et al.
Applied and environmental microbiology, 72(3), 1729-1738 (2006-03-07)
Effects of Quercetin and Rutin on Serum and Hepatic Lipid Concentrations, Fecal Steroid Excretion and Serum Antioxidant Properties
Nakamura Y, et al.
Journal of Health Science, 46(4), 229-240 (2000)
Maksim Rossocha et al.
Biochemistry, 44(15), 5739-5748 (2005-04-13)
Bacterial bile salt hydrolases catalyze the degradation of conjugated bile acids in the mammalian gut. The crystal structures of conjugated bile acid hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex with taurodeoxycholate that was hydrolyzed to the reaction
Anil K Patel et al.
Applied biochemistry and biotechnology, 162(1), 166-180 (2009-08-12)
Probiotic has modernized the current dietetic sense with novel therapeutic and nutritional benefits to the consumers. The presence of bile salt hydrolase (BSH) in probiotics renders them more tolerant to bile salts, which also helps to reduce the blood cholesterol
F Kannenberg et al.
The Journal of biological chemistry, 274(50), 35455-35460 (1999-12-10)
Peroxisomal beta-oxidation plays an important role in the metabolism of a wide range of substrates, including various fatty acids and the steroid side chain in bile acid synthesis. Two distinct thiolases have been implicated to function in peroxisomal beta-oxidation: the
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Protokolle

Enzymatic Assay of Choloylglycine Hydrolase

To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.

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