A2237

Phosphatase, alkalische Garnelen

≥900 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

• Synonym(e): Phosphatase alkalisch aus shrimp
• CAS-Nummer: 9001-78-9
• EC-Nummer: 3.1.3.1 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00131849
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Rekombinant: expressed in proprietary host
• Qualitätsniveau: 200
• Form: buffered aqueous glycerol solution
• Konzentration: ≥900 DEA units/mL
• Fremdaktivität: RNAse, DNAse, none detected
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

Anwendung

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.

Biochem./physiol. Wirkung

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

Einheitendefinition

One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.

Physikalische Form

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl₂

Hinweis zur Analyse

Protein determined by biuret

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable