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A1765

Sigma-Aldrich

S-Acetyl-coenzyme A synthetase from baker′s yeast (S. cerevisiae)

lyophilized powder, ≥3 units/mg protein

Synonym(e):

Acetate CoA ligase (AMP forming), Acetate thiokinase

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About This Item

CAS-Nummer:
9012-31-1
EC-Nummer:
6.2.1.1 (BRENDA, IUBMB)
EG-Nummer:
232-729-7
MDL-Nummer:
MFCD00081267
UNSPSC-Code:
12352204
NACRES:
NA.26

Form

lyophilized powder

Qualitätsniveau

200

Spezifische Aktivität

≥3 units/mg protein

Zusammensetzung

Protein, 10-30% biuret

Lagertemp.

−20°C

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Anwendung

S-Acetyl-coenzyme A synthetase from baker′s yeast (S. cerevisiae) has been used in the synthesis of adenosine 5′-tetraphosphate and adenosine 5′-pentaphosphate.
S-Acetyl-coenzyme A synthetase may be used to study various metabolic pathways, such as glycolysis, gluconeogenesis, pyruvate metabolism and CO2 fixation. It may also be used in gene expression studies.

Biochem./physiol. Wirkung

Acetyl-coenzyme A synthetase catalyzes the production of acetyl-CoA. It is involved in histone acetylation in the nucleus. It may be involved in the growth of nonfermentable carbon sources such as glycerol. Acetyl-coenzyme A synthetase is induced by acetate, acetaldehyde and ethanol .

Verpackung

Package size based on protein content.

Einheitendefinition

One unit will form 1.0 μmole of S-acetyl coenzyme A from acetate, ATP, and coenzyme A per min at pH 7.5 at 37 °C.

Physikalische Form

Lyophilized powder containing stabilizers as potassium phosphate, sucrose, and reduced glutathione

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
SLCL6723
SLCG5103
SLCF8091
SLBW3883
108K1548

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Sigma-Aldrich

C4282

Coenzym A Hydrat

Acetoacetyl-Coenzym A Trinatriumsalz Hydrat cofactor for acyl transfer

Sigma-Aldrich

A1625

Acetoacetyl-Coenzym A Trinatriumsalz Hydrat

Adenosine 5'-tetraphosphate and adenosine 5'-pentaphosphate are synthesized by yeast acetyl coenzyme A synthetase.
Guranowski A, et al.
Journal of Bacteriology, 176(10), 2986-2990 (1994)
Guillaume Baptist et al.
Nucleic acids research, 41(17), e164-e164 (2013-07-31)
We have developed a new screening methodology for identifying all genes that control the expression of a target gene through genetic or metabolic interactions. The screen combines mutant libraries with luciferase reporter constructs, whose expression can be monitored in vivo
Sergio Renilla et al.
Applied microbiology and biotechnology, 93(5), 2109-2124 (2011-09-02)
Impairment of acetate production in Escherichia coli is crucial for the performance of many biotechnological processes. Aerobic production of acetate (or acetate overflow) results from changes in the expression of central metabolism genes. Acetyl-CoA synthetase scavenges extracellular acetate in glucose-limited
Saurabh Sahar et al.
The Journal of biological chemistry, 289(9), 6091-6097 (2014-01-16)
The circadian clock regulates a wide range of physiological and metabolic processes, and its disruption leads to metabolic disorders such as diabetes and obesity. Accumulating evidence reveals that the circadian clock regulates levels of metabolites that, in turn, may regulate
Sara Castaño-Cerezo et al.
Molecular microbiology, 82(5), 1110-1128 (2011-11-09)
Lysine acetylation is a well-established post-translational modification widely conserved and distributed in bacteria. Although multiple regulatory roles have been proved, little is known about its regulation. Here, we present evidence that the transcription of the Gcn5-like acetyltransferase YfiQ of Escherichia
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Artikel

Coenzyme A

Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.

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