A1520

α-Amylase Inhibitor

≥200 inhibitor U/mg protein (using porcine pancreatic α-amylase), ≥1000 inhibitor U/mg protein (using human salivary α-amylase), lyophilized powder

• MDL-Nummer: MFCD00130475
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• product name: α-Amylase Inhibitor from Triticum aestivum (wheat seed), Type I, lyophilized powder
• Biologische Quelle: Triticum estivum
• Qualitätsniveau: 200
• Typ: Type I
• Form: lyophilized powder
• Spezifische Aktivität: ≥1000 inhibitor U/mg protein (using human salivary α-amylase); ≥200 inhibitor U/mg protein (using porcine pancreatic α-amylase)
• Zusammensetzung: Protein, 35-65% biuret
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

α-Amylases are endoglycosidases, that are classified into glycosyl hydrolase family 13 (1-3).

Anwendung

α-Amylase Inhibitor from Triticum aestivum (wheat seed) has been used to determine its effects on salivary gland (SG) amylase activity. It has also been used to inhibit the activity from the extract from Bemisia tabaci.

Biochem./physiol. Wirkung

α-Amylases can hydrolyze α-(1,4)-D-glycosidic linkages. It participates in the breakdown of starch and glycogen. Inhibiting this enzyme can be used as a strategy to treat dental caries, diabetes, obesity and periodontal diseases.

Competitive inhibitor of human salivary α-amylase. K_I = 2.9 nM, compared to a K_M of 5.9 mM (calculated per mole of α-1,4-linked maltose residues).

Einheitendefinition

One unit will reduce the activity of two units of α-amylase (A0521) by 50% after pre-incubation at 25 °C.

Physikalische Form

Lyophilized powder containing buffer salts as sodium phosphate.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)