75543

tris-NTA Biotin -trifluoracetat (Salz) -Lösung

≥80.0% (HPLC)

• Synonym(e): Biotin-tris-NTA trifluoracetic acid salt
• Empirische Formel (Hill-System): C₅₉H₉₃N₁₁O₂₅S
• CAS-Nummer: 1070867-85-4
• Molekulargewicht: 1388.49
• UNSPSC-Code: 12352209
• PubChem Substanz-ID: 329766503
• NACRES: NA.32

Eigenschaften

• Assay: ≥80.0% (HPLC)
• Form: liquid
• Lagertemp.: 2-8°C
• SMILES String: OC(CN(CC(O)=O)[C@H](C(O)=O)CCC(N1CCCN(C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)CCN(C(CCCCCNC(CCCCCNC(CCCC[C@H](SC2)[C@]3([H])[C@@]2([H])NC(N3)=O)=O)=O)=O)CCCN(C(CC[C@@H](C(O)=O)N(CC(O)=O)CC(O)=O)=O)CC1)=O)=O
• InChI: 1S/C59H93N11O25S/c71-43(12-3-1-7-21-61-44(72)13-6-5-11-42-55-38(37-96-42)62-59(95)63-55)60-22-8-2-4-14-45(73)64-23-9-24-66(47(75)19-16-40(57(91)92)69(33-51(81)82)34-52(83)84)29-30-67(48(76)20-17-41(58(93)94)70(35-53(85)86)36-54(87)88)26-10-25-65(28-27-64)46(74)18-15-39(56(89)90)68(31-49(77)78)32-50(79)80/h38-42,55H,1-37H2,(H,60,71)(H,61,72)(H,77,78)(H,79,80)(H,81,82)(H,83,84)(H,85,86)(H,87,88)(H,89,90)(H,91,92)(H,93,94)(H2,62,63,95)/t38-,39-,40-,41-,42-,55-/m0/s1
• InChIKey: ROTBJHGOGUERSI-LNQQGTGFSA-N

Beschreibung

Anwendung

Applications

• Reversible labeling of proteins or cell surfaces
• Detection and analysis of target molecules
• Immobilization of proteins, lipids and cells on surfaces
• Purification and sample preparation of proteins
• Coupling with microscopic or spectroscopic probes (e.g., fluorophores)
• Nickel needs to be added prior application.

Leistungsmerkmale und Vorteile

Features

• A complex of three NiNTA groups ensures high-affinity binding of His-tags
• Binding affinity is approximately four orders of magnitude higher than monovalent metal ion chelators
• Protein binding is stochiometric
• Tris-NTA is available as amino-group or biotin conjugates

Rechtliche Hinweise

This product was produced by Biotechrabbit GmbH. The use of this product for research purposes is covered by a license from QIAGEN Group. No rights to use this product to perform or offer diagnostic, companion diagnostic, commercial testing or other commercial services for money or money′s worth are granted by the supply of this product expressly, by implication or by estoppel.
Should you wish to use this product for any other purpose not covered by this license, please contact QIAGEN Corporate Business Development at bd@qiagen.com.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable